Today’s scientific trends are racing towards smaller scales and experimentation that provides both structural and mechanistic insights. To decipher biomolecular mechanisms you need methods capable of detecting the interactions between proteins and nucleic acids as they happen and at the molecular level.
We offer solutions that enable you to measure, manipulate, and visualize DNA-protein interaction in real-time and at the single-molecule level, with both high throughput and resolution. Uncover the structure, function and dynamicsinterplay between proteins and nucleic acids.
Scientists can use acoustic forces to manipulate, stretch and measure hundreds of single molecules at the same time. Multiple DNA, RNA or protein-coated molecules can be tethered between a bead and a glass surface. Using Acoustic Force Spectroscopy (AFS) technology you can then apply controlled forces on all beads synchronously and probe the mechanical properties of each molecule in parallel. With this highly parallel single molecule method you can:
Scientists can use optical tweezers to trap beads, as depicted at the right, and catch a biomolecule, such as RNA, in between. This biomolecule can then be manipulated by moving the beads, while the force and extension are measured. Fluorescently labeled proteins can be visualized with confocal or STED fluorescence microscopy. The combination of optical tweezers with simultaneous multicolor fluorescence measurements allows correlating the mechanical properties of the RNA with the protein activity. With optical tweezers – fluorescence microscopy you can: