Application Note Archives - LUMICKS

The interplay between dynamics, structure, and function of many RNA molecules is tied to essential biological processes, such as the regulation of gene expression and protein synthesis. However, modern imaging and biochemical methods often fail in explaining these relations. This application note is a complete guide on how to resolve the structure-function relationship…

The cytoskeleton and its many molecular motors are major players in a variety of cellular functions. The interconnected networks of cytoskeletal filaments act as tracks directing molecular motor transport and provide structural support and organization to cells. One example of a class of motor proteins is the myosin superfamily, a…

DNA breathing, also known as fraying, describes the spontaneous local conformational transitions occurring within double-stranded DNA (dsDNA). Due to the transient and spontaneous nature of this process, the study of real-time DNA breathing transitions at the single-molecule level requires the use of highly sensitive experimentation technologies. Using LUMICKS’ high-resolution optical tweezers, small and transient DNA…

Protein filaments have been studied indirectly using various approaches of fluorescence microscopy in cells and tissues and more directly, using force spectroscopy on single filaments or filament bundles. LUMICKS' C-Trap™ provides for a unique approach which combines the strength of both techniques, to give a truly correlative method of studying…