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Study using CTFM Published in Nature Communications

optical-tweezers-fluorescence-microscopy-Nature-Communications

A new paper using the correlative optical tweezers-fluorescence microscopy (CTFM) technology has recently been published in Nature Communications. King, Peterman & Wuite show in “Unravelling the structural plasticity of stretched DNA under torsional constraint” that in torsionally constraint DNA concomitant basepair melting and helical unwinding can occur at forces >~50 pN. They also show that an overwound DNA-structure is created at forces >~110 pN. Read the full study here.

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