The characterization of the enzymatic activity of proteins involved in the DNA machinery and the identification of their inhibitors are key for the development of a variety of treatments. It is essential to collect statistically-relevant data, which is typically achieved by using enzymatic assays designed for high-throughput screening applications. However, while these assays provide valuable insights into the bulk characteristics of enzymes, they suffer from the limitation that the unique behavior of each molecule is averaged out. Acoustic force spectroscopy has the unique ability to measure the activity of hundreds of enzymes in parallel, while retaining valuable information, such as the processivity, rate and pausing steps of each individual enzyme.
The Enzymatic Activity and Inhibition Efficiency application note shows how LUMICKS’ AFS™ system can be employed for the study of DNA transcription and the detection of novel transcription elongation inhibitors.
Download the application note here.