Drug Research

Part of: Applications

It is possible to prevent noneffective drugs from entering costly (pre-)clinical trials by gaining a better understanding of the underlying biological mechanisms of drug-protein targets and the mechanism of action of promising leads. We offer solutions that enable to directly observe complex dynamic molecular processes and identify the most promising leads.

Visualize and measure DNA-protein interactions in real-time at the single-molecule level

with: Optical Tweezers and Fluorescence Microscopy

Scientists can study how proteins interact with DNA or RNA molecules and visualize these interactions while correlating them with the mechanical properties of the system. In this way, they can characterize not only protein binding but also binding location and protein behavior after the binding, thus resolving the dynamics and functionality of the target. This additional information can help interpret the influence of different pharmaceuticals on the protein-DNA interactions to discover promising leads based on their MOA.

  • Achieve better target validation and understanding of the druggable mechanism of action early in the process by investigating the complex dynamic molecular mechanisms of DNA-protein interactions
  • Visualize and measure the dynamics of DNA-protein interactions under the influence of pharmaceuticals to gain insight into the drug’s mechanism of action
protein DNA interactions drug discovery
Learn more about:
Technology
Optical Tweezers and Fluorescence Microscopy

Study protein conformational changes in real-time at the single-molecule level

with: Optical Tweezers and Fluorescence Microscopy

Scientists can use optical tweezers to trap beads and catch a biomolecule, such as a protein, in between. The folding and unfolding of the protein can then be monitored by moving the beads while measuring the force and extension. The combination of optical tweezers with simultaneous multicolor fluorescence measurements (e.g. with FRET) allows correlating the global mechanical properties of the protein with the local structural properties. With optical tweezers – fluorescence microscopy you can:

  • Study the mechanism behind the biological function of proteins by looking at theconformational changes of protein targets, in real-time and with Ångström resolution
  • Observe conformational changes of protein targets in the presence of different pharmaceuticals. Find out whether the desired mechanism(s) of action is fulfilled by the drug in a quick, easy and effective manner
C-Trap Protein Folding
Learn more about:
Technology
Optical Tweezers and Fluorescence Microscopy

Study Drug-Molecule Interactions at the Nanoscale

with: Optical Tweezers and Fluorescence Microscopy

Scientists can use optical tweezers to trap beads and catch a biomolecule, such as DNA, in between, while an enzyme is interacting with it. It is then possible to study and quantify the effect of small molecules or biologics on the enzyme’s activity. The combination of optical tweezers with simultaneous multicolor fluorescence measurements allows correlating the mechanical properties of the DNA with the drug activity. With optical tweezers – fluorescence microscopy you can:

  • Study the binding properties of small molecules and biologics and visualize their interactions with biomolecules, such as motor proteins and DNA, in real-time
  • Directly visualize transient multi-step processes and observe at which step inhibition occurs and how
  • Relate structural information with specific drug binding
  • Precisely screen for small molecules and biologics, quickly and effectively
  • Perform experiments under biologically relevant conditions and highly crowded environments and link the in vitro experiment with the in vivo situation
C-Trap Small Molecule Protein Interaction
Learn more about:
Technology
Optical Tweezers and Fluorescence Microscopy
Optical Tweezers and Fluorescence Microscopy

Technology

The combination of optical tweezers and fluorescence microscopy allows for simultaneous manipulation and visualization of molecular interactions in real-time.

Solutions

C-Trap® Optical Tweezers Fluorescence & Label-free Microscopy
C-Trap

Optical Tweezers and Fluorescence Microscopy

M-Trap® Optical Tweezers Fluorescence & Label-free Microscopy
m-Trap

Optical Tweezers