NK cells from donor 2 induced 3-fold higher cytotoxicity compared with NK cells from donor 1 (74% and 24%, respectively). NK cells from donor 2 required higher forces than those from donor 1 to detach from target cells (Figure 5).
The data suggest that cell avidity is a reliable predictor of NK cell function and that cell avidity analyses can be applied to different types of immune cells.
5 Left: Cytotoxic effect of NK cells from donor 1 and donor 2 on the same population of target cells. Right: Avidity curve showing the proportion of target-bound NK cells upon application of a force ramp. (rForce indicates relative force).