Identify the Goldilocks CAR: Cell avidity as a parameter for optimal CAR-antigen combinations

Part of: CAR-T cells

Identify the Goldilocks CAR: Cell avidity as a parameter for optimal CAR-antigen combinations

This study aimed to identify CAR T cells that bind high antigen-expressing targets but not low antigen-expressing cells.

Researchers validated low functioning and high functioning CAR T cells based on functional assays and used non-transduced T cells as a negative control (NC). T cell avidities were tested on adherent cells with low antigen expression, high antigen expression, or no antigen expression.

The cell avidity data correlated with a corresponding in vitro cell killing assay, showing antigen-dependent binding strengths and cell killing, respectively (Figure 1). The cell-killing results also demonstrated significantly higher toxicity induced by high functioning CAR T cells on low antigen-expressing healthy-like cells (Figure 2).

The z-Movi® can distinguish between T cell specificities to improve CAR T cell therapy. Cell avidity is a reliable readout for identifying optimal CARs that can minimize on-target/off-tumor activity by CAR T cells.

1 Avidity scores based on the average detaching forces of the CAR T-cell populations relative to the non-transduced T-cell control population.

2 Cytotoxic effect of high and low functionality CARs on target cells with different antigen expression levels.

Data courtesy of Dr. Steven Albelda at University of Pennsylvania

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z-Movi® is the only instrument that enables you to identify the most potent immune cells and quantify their binding strength to their target. All this in a label-free and single-cell manner.

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